Essendon has advised Heath Hocking he will not be offered a contract for 2018.
The 29-year-old has played 126 games for the Bombers across a 12-year career, including two this season.
He finished runner up in the Crichton Medal in 2010, and was named Best Team Player in 2014.
Essendon's general manager of football Rob Kerr thanked Hocking for his time at the club.
"Heath has been a wonderful player and highly respected Essendon person over a long period of time,” Kerr said.
"Drafted as a rookie, Heath earned his spot on the senior list and went on to become a 100 game player for the club.
"He is a true competitor, tough, uncompromising and the ultimate professional, on and off the field.
"On behalf of everyone at the club we wish Heath all the very best for the future."
Hocking said the journey has been "amazing".
"I want to thank the football club for the last 12 years. It’s been an amazing time for me," he said.
"I feel as though I’m a better person for being involved with the footy club.
"Thank you to my teammates and coaches. I have some great memories and I’ve met so many great people, who I will have friendships with for life.
"I’m sad that it has come to an end but I’m excited for the future and what that holds."