A magnitude 3.2 quake rattled Adelaide about 8.30pm on Wednesday local time.
The quake was recorded at Mannum in the Murray Darling region at a depth of 12km, according to Geoscience Australia.
Locals reported a tremor that rattled windows.
"I felt the windows of my house shake in Payneham," senior seismologist David Love told the ABC.
"We wondered quite what it was.
"It was just a gentle rattling of windows, no sound of any plane or anything, and I thought 'oh it's going on a bit long for an earthquake'."